Wheat PDI family protein
|
Content (pmol/mg protein)
|
---|
5 dpa
|
10 dpa
|
15 dpa
|
25 dpa
|
30 dpa
|
35 dpa
|
Mature
|
flour
|
---|
TaPDIL1 | 15.0 | 27.4 | 23.2 | 6.9 | 3.4 | 2.9 | 4.3 | 7.3 |
TaPDIL2 | 3.9 | 3.8 | 2.7 | 0.5 | 0.6 | 0.4 | 0.6 | 1.1 |
TaPDIL3 | 0.7 | 0.3 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
TaPDIL4 | 12.7 | 11.6 | 12.1 | 4.1 | 3.2 | 3.0 | 3.5 | 1.5 |
TaPDIL5 | 1.5 | 1.3 | 1.2 | 0.3 | 0.2 | 0.2 | 0.3 | 0.3 |
- Contents by weight of PDI family proteins were estimated from the band intensities on western blot analysis of the caryopses (Figure 4B) and flour (Haruyokoi) with recombinant TaPDIL1Aα, TaPDIL2, TaPDIL3A, TaPDIL4D, and TaPDIL5A used as standard proteins for determination of TaPDIL1, TaPDIL2, TaPDIL3, TaPDIL4, and TaPDIL5, respectively. Molar quantities of TaPDIL1, TaPDIL2, TaPDIL3, TaPDIL4, and TaPDIL5 included in 1 mg of caryopses protein or flour protein were calculated with each molecular weight of TaPDIL1Aα, TaPDIL2, TaPDIL3A, TaPDIL4D, and TaPDIL5A (as shown in Table 2), respectively.