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Table 4 PDI family proteins in wheat caryopses and flour

From: Expression and characterization of protein disulfide isomerase family proteins in bread wheat

Wheat PDI family protein Content (pmol/mg protein)
5 dpa 10 dpa 15 dpa 25 dpa 30 dpa 35 dpa Mature flour
TaPDIL1 15.0 27.4 23.2 6.9 3.4 2.9 4.3 7.3
TaPDIL2 3.9 3.8 2.7 0.5 0.6 0.4 0.6 1.1
TaPDIL3 0.7 0.3 0.2 0.1 0.1 0.1 0.1 0.1
TaPDIL4 12.7 11.6 12.1 4.1 3.2 3.0 3.5 1.5
TaPDIL5 1.5 1.3 1.2 0.3 0.2 0.2 0.3 0.3
  1. Contents by weight of PDI family proteins were estimated from the band intensities on western blot analysis of the caryopses (Figure 4B) and flour (Haruyokoi) with recombinant TaPDIL1Aα, TaPDIL2, TaPDIL3A, TaPDIL4D, and TaPDIL5A used as standard proteins for determination of TaPDIL1, TaPDIL2, TaPDIL3, TaPDIL4, and TaPDIL5, respectively. Molar quantities of TaPDIL1, TaPDIL2, TaPDIL3, TaPDIL4, and TaPDIL5 included in 1 mg of caryopses protein or flour protein were calculated with each molecular weight of TaPDIL1Aα, TaPDIL2, TaPDIL3A, TaPDIL4D, and TaPDIL5A (as shown in Table 2), respectively.