Table 2 Characteristics of the wheat PDI family proteins cloned in this study
From: Expression and characterization of protein disulfide isomerase family proteins in bread wheat
Name | Total amino acid residues | Signal peptide* | Molecular weight | pI | Conserved active site | Consensus N-glycosylation site(s) | Conserved arginine(s) | Conserved charge pairs | ER retention signal |
|---|---|---|---|---|---|---|---|---|---|
TaPDIL1Aα | 515 | 1–25 | 56579.13 | 4.99 | C68GHC71, C412GHC415 | N283 | R136, R475 | E62/K96, E406/K439 | KDEL |
TaPDIL1Aβ | 501 | 1–26 | 55256.73 | 5.07 | C54GHC57, C398GHC401 | N269 | R122, R461 | E48/K82, E392/K425 | KDEL |
TaPDIL1Aγ | 487 | 1–18 | 53977.23 | 5.10 | C40GHC43, C384GHC387 | N255 | R108, R447 | E34/K68, E378/K411 | KDEL |
TaPDIL1Aδ | 485 | 1–16 | 53679.83 | 5.10 | C38GHC41, C382GHC385 | N253 | R106, R445 | E32/K66, E376/K399 | KDEL |
TaPDIL1B | 512 | 1–25 | 56425.02 | 5.03 | C68GHC71, C412GHC415 | N283 | R136, R475 | E62/K96, E406/K439 | KDEL |
TaPDIL2 | 585 | 1–28 | 63588.76 | 4.61 | C129GHC132, C470GHC473 | N109, N212 | R136, R535 | E123/K157, E464/K497 | KDEL |
TaPDIL3A | 541 | 1–23 | 59594.01 | 4.95 | C96ERS99, C435VDC438 | N150 | R160 | E429K462 | KDEL |
TaPDIL4D | 367 | 1–30 | 40260.95 | 6.17 | C60GHC63, C179GHC182 | - | R125, R244 | E54/K87, E173/K211 | - |
TaPDIL5A | 440 | 1–22 | 47207.89 | 5.30 | C57GHC60, C194GHC197 | N164, N170 | R119, R257 | E51/K89, E188/K226 | NDEL |