WRKY transcription factors that are not from higher plants. A. Bootstrap consensus tree (1,000 replicates) of a Neighbor Joining phylogenetic tree derived from a MUSCLE alignment of WRKY domains from the species described in Figure 2. Shown is a non-higher plant clade that contains algae (green), diplomonad (blue), and amoebozoa (black) WRKY transcription factors. B. Phylogenetic tree of Group I-like WRKY proteins from social amoebae and other amoebozoa. Groups I CTD, I NTD, IIc and an intermediate clade are shown. Unicellular green algae WRKY domains are marked with a green dot, amoebozoa black, social amoebae purple and Klebsormidium flaccidum in orange.