Parallel action of AtDRB2 and RdDM in the control of transposable element expression

Table 1 Mass spectrometric analysis of DRB2-Cmyc affinity purification

In gel	Protein	AGI code	Score	Coverage (%)	Unique peptides	SC	emPAI
55 < 72	DRB2	At2g28380	1372.8	59.2	18	31	6.32
	PRMT4B	At3g06930	668.1	26.9	9	11	0.76
	MSI4	At2g19520	532.8	28.3	8	10	0.73
	WD40 containing	At3g18060	370.1	20.4	8	8	0.45
	NUCL1	At1g48920	358.8	12	6	6	0.37
	HDA19	At4g38130	339.7	11.2	4	4	0.24
	SWI3A	At2g47620	200	7.9	3	3	0.17
	MBD10	At1G15340	195.3	15.6	5	5	0.43
	WD40 containing	At5g24710	115.1	1.4	2	2	0.04
	WD40 containing	At2g01330	87.7	6	2	2	0.13
<55	NFA03	At5g56950	287.5	12.7	5	5	0.42
	PRL1	At4g15900	228.5	15.7	5	5	0.33
	AKIN10	At3g01090	103.1	6.6	3	3	0.17
	WD40 containing	At1g04510	82.1	4.7	2	2	0.11
95 < 130	AGO4	At2g27040	172.6	4.1	4	4	0.13
>250	ACC1	At1g36160	1959.2	23	43	43	0.7
	WD40 containing	At2g21390	568.5	10.3	11	11	0.28
	AGO4	At2g27040	167.1	4.7	4	4	0.13
	CAND1	At2g02560	117.8	3.1	3	3	0.07
	TPL	At1g15750	113.2	4.7	5	5	0.13

Table summarizing the multiple proteins specifically found in the DRB2-CMyc purified extract. Proteins found in one cut band are grouped, and all are ordered by their respective scores. Protein names as well as the corresponding AGI codes, the score of each protein, the percent coverage for the known protein sequence and the number of unique peptides matching to the protein are given. The spectral count (SC) is the total number of sequenced peptides for a protein, and the exponentially modified protein abundance index (emPAI) (defined as 10^{Nobserved/Nobservable} – 1, were N is either the number of observed peptides or the number of theoretically observed peptide after trypsin digestion), is indicated to estimate the abundance of a given protein in an extract [66].