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Table 1 Mass spectrometric analysis of DRB2-Cmyc affinity purification

From: Parallel action of AtDRB2 and RdDM in the control of transposable element expression

In gel Protein AGI code Score Coverage (%) Unique peptides SC emPAI
55 < 72 DRB2 At2g28380 1372.8 59.2 18 31 6.32
  PRMT4B At3g06930 668.1 26.9 9 11 0.76
  MSI4 At2g19520 532.8 28.3 8 10 0.73
  WD40 containing At3g18060 370.1 20.4 8 8 0.45
  NUCL1 At1g48920 358.8 12 6 6 0.37
  HDA19 At4g38130 339.7 11.2 4 4 0.24
  SWI3A At2g47620 200 7.9 3 3 0.17
  MBD10 At1G15340 195.3 15.6 5 5 0.43
  WD40 containing At5g24710 115.1 1.4 2 2 0.04
  WD40 containing At2g01330 87.7 6 2 2 0.13
<55 NFA03 At5g56950 287.5 12.7 5 5 0.42
  PRL1 At4g15900 228.5 15.7 5 5 0.33
  AKIN10 At3g01090 103.1 6.6 3 3 0.17
  WD40 containing At1g04510 82.1 4.7 2 2 0.11
95 < 130 AGO4 At2g27040 172.6 4.1 4 4 0.13
>250 ACC1 At1g36160 1959.2 23 43 43 0.7
  WD40 containing At2g21390 568.5 10.3 11 11 0.28
  AGO4 At2g27040 167.1 4.7 4 4 0.13
  CAND1 At2g02560 117.8 3.1 3 3 0.07
  TPL At1g15750 113.2 4.7 5 5 0.13
  1. Table summarizing the multiple proteins specifically found in the DRB2-CMyc purified extract. Proteins found in one cut band are grouped, and all are ordered by their respective scores. Protein names as well as the corresponding AGI codes, the score of each protein, the percent coverage for the known protein sequence and the number of unique peptides matching to the protein are given. The spectral count (SC) is the total number of sequenced peptides for a protein, and the exponentially modified protein abundance index (emPAI) (defined as 10Nobserved/Nobservable – 1, were N is either the number of observed peptides or the number of theoretically observed peptide after trypsin digestion), is indicated to estimate the abundance of a given protein in an extract [66].