Figure 6

Mutation of the DAG2 gene affects GA biosynthesis. Relative expression level of AtGA3ox1, AtGA3ox2 and AtGA2ox2 (A), and of ABA1, ABA2, NCED6, NCED9 and CYP707A2 (B) in dag2 mutant seeds compared to wild-type seeds. Seeds were imbibed 12 hours in the dark (D), or under R light (R). Relative expression levels were normalized with that of the UBQ10 gene, and are presented by the ratio of the corresponding wild-type mRNA level in D, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05).