expression is regulated by RGA and GAI. Relative expression level of: DAG2 in rga28
(A), and gai-t6 mutant seeds (B), and of RGA
(C) or GAI
(D) in dag2 mutant seeds, compared to wild-type seeds. Seeds were imbibed 12 hours in the dark (D), or under R light (R). Relative expression levels were normalized with that of PP2A (At1g13320) (A, B), or of UBQ10
(C, D), and are presented by the ratio of the corresponding wild-type mRNA level in D, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05).