Figure 2

DAG2 expression is induced by imbibition. Relative expression level of DAG2 in wild-type dry seeds (0 h), or imbibed 12 (12 h) or 24 hours (24 h) in the dark (D) or under White (W) or Red (R) light (A). Relative expression levels were normalized with that of the ACTIN2 (At3g18780) gene, and are presented by the ratio of the corresponding mRNA level in dry seeds, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05). Histochemical staining of DAG2:GUS dry seeds, or imbibibed 12 hours under W light (W) or in the dark (D) (B).