Figure 1

Mutation of DAG2 affects seed germination under R light. Relative expression level of DAG2 in wild-type seeds imbibed 24 hours in the dark (D), or under phyB-dependent conditions, (A), and in the dark or under phyA-dependent conditions (B). Relative expression levels were normalized with that of the UBQ10 (At4g05320) gene, and are presented by the ratio of the corresponding mRNA level in Dark, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values.Germination rates of wild-type and dag2 mutant seeds, grown 5 days under phyB-dependent germination conditions (C). Error bars = SEM. The diagram at top depicts the light treatment scheme for the experiment. FRp, Far Red pulse (40 μmol m⁻² s⁻¹); Rp, Red pulse (90 μmol m⁻² s⁻¹). Significative differences were analyzed by t-test (*P ≤ 0,05).