Table 1 Statistics of sRNAs (small RNA) sequences from the individual libraries
Category | S-0 h (%*) | R-0 h (%*) | S-12 h (%*) | R-12 h (%*) | S-7 d (%*) | R-7 d (%*) |
|---|---|---|---|---|---|---|
Total raw reads | 13,469,920 (100) | 14,055,204 (100) | 13,022,329 (100) | 15,082,285 (100) | 15,458,638 (100) | 16,720,995 (100) |
High quality reads | 13,182,326 (97.865) | 13,760,385 (97.902) | 12,789,856 (98.215) | 14,806,386 (98.171) | 15,157,737 (98.054) | 16,328,908 (97.655) |
3′ adaptor null reads | 41,860 (0.311) | 41,310 (0.294) | 33,666 (0.259) | 44,797 (0.297) | 46,888 (0.303) | 56,998 (0.341) |
Insert null reads | 3,031 (0.023) | 1,505 (0.011) | 2,756 (0.021) | 2,639 (0.018) | 4,029 (0.026) | 2,549 (0.015) |
5′ adaptor contaminant reads | 9,566 (0.071) | 8,254 (0.059) | 6,932 (0.053) | 12,279 (0.081) | 8,836 (0.057) | 34,253 (0.205) |
Smaller than 18 nt reads | 84,194 (0.625) | 192,261 (1.368) | 49,530 (0.380) | 60,857 (0.404) | 82,752 (0.535) | 185,583 (1.110) |
Poly(A) sequence reads | 269 (0.002) | 447 (0.003) | 485 (0.004) | 1,678 (0.011) | 308 (0.002) | 812 (0.005) |
Total clean sRNA reads | 13,043,406 (96.834) | 13,516,608 (96.168) | 12,696,487 (97.498) | 14,684,136 (97.360) | 15,014,924 (97.130) | 16,048,713 (95.979) |
Unique sequence reads | 5,692,793 (42.263) | 4,919,934 (35.004) | 5,245,389 (40.280) | 5,250,004 (34.809) | 5,624,720 (36.386) | 5,139,762 (30.738) |
Singleton sequence reads | 4,503,775 (33.436) | 3,841,747 (27.333) | 4,033,830 (30.976) | 3,959,027 (26.250) | 4,392,276 (28.413) | 3,881,932 (23.216) |
Unique sequence reads (>2 reads) | 1,189,018 (8.827) | 1,078,187 (7.671) | 1,211,559 (9.304) | 1,290,977 (8.560) | 1,232,444 (7.973) | 1,257,830 (7.523) |