|
Category
|
S-0 h (%*)
|
R-0 h (%*)
|
S-12 h (%*)
|
R-12 h (%*)
|
S-7 d (%*)
|
R-7 d (%*)
|
|---|
|
Total raw reads
|
13,469,920 (100)
|
14,055,204 (100)
|
13,022,329 (100)
|
15,082,285 (100)
|
15,458,638 (100)
|
16,720,995 (100)
|
|
High quality reads
|
13,182,326 (97.865)
|
13,760,385 (97.902)
|
12,789,856 (98.215)
|
14,806,386 (98.171)
|
15,157,737 (98.054)
|
16,328,908 (97.655)
|
|
3′ adaptor null reads
|
41,860 (0.311)
|
41,310 (0.294)
|
33,666 (0.259)
|
44,797 (0.297)
|
46,888 (0.303)
|
56,998 (0.341)
|
|
Insert null reads
|
3,031 (0.023)
|
1,505 (0.011)
|
2,756 (0.021)
|
2,639 (0.018)
|
4,029 (0.026)
|
2,549 (0.015)
|
|
5′ adaptor contaminant reads
|
9,566 (0.071)
|
8,254 (0.059)
|
6,932 (0.053)
|
12,279 (0.081)
|
8,836 (0.057)
|
34,253 (0.205)
|
|
Smaller than 18 nt reads
|
84,194 (0.625)
|
192,261 (1.368)
|
49,530 (0.380)
|
60,857 (0.404)
|
82,752 (0.535)
|
185,583 (1.110)
|
|
Poly(A) sequence reads
|
269 (0.002)
|
447 (0.003)
|
485 (0.004)
|
1,678 (0.011)
|
308 (0.002)
|
812 (0.005)
|
|
Total clean sRNA reads
|
13,043,406 (96.834)
|
13,516,608 (96.168)
|
12,696,487 (97.498)
|
14,684,136 (97.360)
|
15,014,924 (97.130)
|
16,048,713 (95.979)
|
|
Unique sequence reads
|
5,692,793 (42.263)
|
4,919,934 (35.004)
|
5,245,389 (40.280)
|
5,250,004 (34.809)
|
5,624,720 (36.386)
|
5,139,762 (30.738)
|
|
Singleton sequence reads
|
4,503,775 (33.436)
|
3,841,747 (27.333)
|
4,033,830 (30.976)
|
3,959,027 (26.250)
|
4,392,276 (28.413)
|
3,881,932 (23.216)
|
|
Unique sequence reads (>2 reads)
|
1,189,018 (8.827)
|
1,078,187 (7.671)
|
1,211,559 (9.304)
|
1,290,977 (8.560)
|
1,232,444 (7.973)
|
1,257,830 (7.523)
|
- * The ratio is equal to the separate reads divided by the total raw reads.
- S-0 h, S-12 h, and S-7 d represent shoot treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively. R-0 h, R-12 h, and R-7 d represent root treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively.
