Validation of expression profiles of miRNAs. (A) Heat map of sequencing (S) and stem-loop qRT-PCR data (P). Relative expression level was calculated using Log2(Salt/Salt-0 h), and qRT-PCR data were averaged using the results from three technical repeats to represent three independent experiments. (B) Scatterplot of miRNA expression showing the correlation between deep sequencing (DS) and qRT-PCR (qRT) results. S-0 h, S-12 h, and S-7 d represent the shoots treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively. R-0 h, R-12 h, and R-7 d denote the roots treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively.