Figure 3

Differentially expressed S. europaea miRNAs after salt treatment or between the shoots and roots. A to E, Heat map of differentially expressed S. europaea miRNAs after salt treatment. These miRNAs were divided into five categories based on their expression patterns. (A) miRNAs down-regulated in salt-treated shoots. (B) miRNAs down-regulated in salt-treated roots. (C) miRNAs down-regulated in salt-treated shoots and roots. (D) miRNAs up-regulated by salt. (E) miRNAs dynamically regulated during salt treatment. Relative expression level was calculated using Log₂(RPM_Salt/RPM_{salt-0 h}). F to H, Heat map of differentially expressed S. europaea miRNAs between the shoots and roots. (F) miRNAs expressed higher in the roots than those in the shoots. (G) miRNAs expressed higher in the shoots than those in in the roots. (H) miRNAs distributed dynamically between the shoots and roots during salt treatment. Relative expression level was calculated with Log₂(RPM_root/RPM_shoot). S-0 h, S-12 h, and S-7 d represent the shoots treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively. R-0 h, R-12 h, and R-7 d denote the roots treated with 200 mM NaCl for 0 h, 12 h, and 7 d, respectively.