Averaged transcript levels of four genes in transgenic
plants. X-axis depicts the transgenic lines and control plants (where n = number analysed). The transcript values were averaged for sibling lines with similar phenotype. Where possible, null segregants were selected from three different parental lines. For clarity between very high and low transcript levels, the Y-axis for normalised mRNA copies/microlitre is divided into two different scales (black and red). Error bar is the standard error of the mean (SEM) of biological variation between sibling lines. (A) Transcripts measured for SCW 35S:HvCesA4 transgenic plants were the HvCesA4 transgene and eHvCesA4, eHvCesA7 and eHvCesA8. Plants within the same line exhibited variations in phenotype. There were three independent lines with a dwarfed phenotype (black solid circle) and three other with a normal phenotype. (B) Transcripts measured for SCW 35S:HvCesA8 transgenic plants were the HvCesA8 transgene and eHvCesA4,eHvCesA7 and eHvCesA8. There were three lines that were stunted, sterile and died young (open circle), three lines with a “brittle node” phenotype (black solid circle) and three lines with a normal phenotype.