Lignification and suberization of cortical parenchymatous cells. A: Cross section of the lateral margin of a fenestration wound, 8 days after wounding, stained with safranine and alcian blue and seen in bright-field microscopy. Cortical parenchymatous cells in the border got lignified (arrow). B-C: Lateral edge of the wound seen by bright-field (B) and fluorescence microscopy (C), stained with phloroglucinol-HCl. Protective barrier of lignified (arrow) and suberized (arrowhead) parenchymatous cells in the cortex. Reddish staining under white light reveals the presence of lignin, while higher fluorescence intensity under UV light corresponds to suberin deposits in cell walls.