Figure 5

Channel behavior of PsOep23 in presence of spermine. Current trace of a single active Oep23 channel at a holding potential of (A) +100 and (B) -100 mV in control conditions and after addition of 5 and 10 mM of spermine, respectively. The presence of spermine induced a concentration dependent reduction of the open channel conductance (o - > o’) and the open probability, apparent by a more frequent and prolonged likelihood to remain in a closed or blocked state. This block is completely reversible by washing out spermine (data not shown).