Figure 2

PsOep23 temporarily fully opens. Current trace of a bilayer containing a single active Oep23 channel at a holding potential of +100 mV. Apart from the main open (o-main) and the sub-conductance state (o-sub) the channel sometimes switched to its fully open state (o-full) and especially at holding potentials above +/−100 mV to the closed state (c). Electrolyte solution 250 mM KCl, 50 mM Mops/Tris pH 7.0 (symmetrical cis/trans).