Construct
|
Gene
|
Subcellular localization
|
Total plants
|
Number of events
|
Expression level T1
|
Expression level T2*
|
---|
TrCel5A
ER
|
AAA34213.1
|
ER
|
50
|
39
|
15.9 ± 11.8
|
40.8 ± 5.2
|
TrCel5A
AP
|
AAA34213.1
|
Apoplast
|
35
|
13
|
9.4 ± 7.5
|
29.9 ± 6.4
|
- Events and plants that produced seed from vectors designed to express endoglucanase from T. reesei. The mean value of expression level [nmol 4MU min−1 mg−1] of all positive plants for each construct is shown. *Values for second-generation represents the mean of three homozygous lines (T2) with ten samples each are also shown.