Transgenic tobacco plants expressing TrCel5A. Eight-week-old N. tabacum SR1 wt plants were grown in soil under standard conditions. Twenty plants of each line and wt were monitored on their stem size TrCel5AAP plants showed a significant reduction in size and growth rate, whereas TrCel5AER plants were not significantly different from wt (D). The leaf shape of several transgenic plants was altered compared to wt (C). TrCel5AER
(A) and TrCel5AAP plants (B) displayed a curly leaf phenotype which in the case of TrCel5AAP plants included additional necrotic lesions.