Figure 2

Localization of TrCel5A in transgenic tobacco leaf tissue. Plant tissue expressing TrCel5A targeted to the ER (A) and wt (B) were immunostained with the MαKDEL primary antibody and GAM_FC ^FITC. Apoplast targeting was monitored by staining transgenic plant tissue and wt with RαCell primary antibody and GAR_FC ^FITC (C-D). Electron microscopy with immunolabeling (RαCell/GAR_FC ^Gold) indicated localization for TrCel5A_AP inside the apoplastic space or at the cell walls (E, G) and for TrCel5A_ER inside the ER (F , H). Arrows mark accumulation of recombinant enzyme.