Localization of TrCel5A in transgenic tobacco leaf tissue. Plant tissue expressing TrCel5A targeted to the ER (A) and wt (B) were immunostained with the MαKDEL primary antibody and GAMFC
FITC. Apoplast targeting was monitored by staining transgenic plant tissue and wt with RαCell primary antibody and GARFC
(C-D). Electron microscopy with immunolabeling (RαCell/GARFC
Gold) indicated localization for TrCel5AAP inside the apoplastic space or at the cell walls (E, G) and for TrCel5AER inside the ER (F
H). Arrows mark accumulation of recombinant enzyme.