Generation of transgenic tobacco expressing TrCel5A. Genomic PCR of DNA prepared from wt and transgenic plants with primers binding in the CaMV 35SS expression cassette and below genomic PCR with primers binding in a highly conserved region in the chloroplast genome  (A); M, GeneRuler™ 1 kb wt: SR1 wild type, nc: H2O, pc: control plasmid DNA pTRAkc-TrCel5A-ER. Western Blot for recombinant TrCel5A from three different TrCel5AAP and TrCel5AER lines (B). Lanes contain 10 μg of TSP from transgenic plants; Antibody system used in is MαHis5 /GAMFc
AP; wt: SR1 wild type, pc: purified His6 tagged Phenylammonium lyase from Zea mays (ZmPAL-His6). In all transgenic lines the degraded TrCel5A is detectable. Additionally in TrCel5AER lines also small amounts of the full enzyme are detectable. Expression level of TrCel5A in transgenic tobacco lines was determined by conversion of 4MUC (C). Three independent lines with five plants each were tested (coloured bars). White bars represent the average expression level in TrCel5AAP and TrCel5AER lines respectively.