2-BP interfered with vacuolar trafficking. A-D. 4 DAG WT seedlings were pulse-labeled with FM4-64, washed and incubated for 30 min (A), or pulse-labeled with FM4-64 followed by 30 min incubation with 1/2 MS medium supplemented with 50 μM BFA (B). BFA-treated seedlings were then washed with 1/2 MS medium supplemented with either DMSO (BFA wo + DMSO) (C) or 2-BP (BFA wo + 2-BP) (D). Arrows point at the tonoplat labeled by FM4-64. Arrowhead indicates the BFA compartment. Results are representative of 20 root hairs for each treatment. Bars = 7.5 μm.