2-BP abolished the tonoplast localization of CBL2 in root hairs. A-D. 4 DAG seedlings of Pro
:CBL2-RFP transgenic plants treated with either DMSO (A, B) or 2-BP (C, D) for 4–12 hr before visualization. E-F. 4 DAG seedlings of Pro
:CBL2-RFP;pat10-2 transgenic plants treated with DMSO for 4–12 hr before imaging. Representative initiating (A, C, E) or elongating (B, D, F) root hairs are shown. V indicates vacuole. G. Quantification of CBL2-RFP distribution in the tonoplast v.s. the cytoplasm (Tonoplast/Cyt) at different time points after 2-BP treatment. a.u. stands for arbitrary fluorescence units. Bars = 7.5 μm.