Hormone level of abscisic acid (ABA) (A), and the expression levels of ABA biosynthesis gene,
(B) and ABA-responsive gene,
(C) in the pericarp of low and high seed weight-to-berry weight (SB) groups. ABA levels were quantified by LC-MS/MS using four replicates. Gene expressions were measured by qRT-PCR and expression levels are relative to those of low-SB berries at 2-wk PV (n = 5). Error bars indicate ± SEM. Prevéraison stages were two- and one-week before véraison (2-wk PV and 1-wk PV). Gene expressions were also measured at mid-véraison (MV) cluster stage. Significant differences between low- and high-SB berries at each cluster stage are indicated by asterisks (Student’s t-test, p < 0.05). Significant differences of each SB group between the ripening stages are denoted by different letters (lower and upper case letters are used for low- and high-SB berries, respectively (Tukey’s HSD, p < 0.05)).