A Matlab histograms of voxel intensity distribution for each ROI, TW, OW and NW and 3D render of each ROI, units are not included as the number of voxels varied (histograms are to compare intensity distribution). Each tree, RW induced (T1, T2 and T3) or controls (C1, C2 and C3) were scanned including a common internal standard – allowing comparison of average voxel intensity. B Average ROI voxel intensity. Error bars = standard error of tissue type across 3 trees. * p < 0.05 (one-way ANOVA).