The contrasting N management of two oilseed rape genotypes reveals the mechanisms of proteolysis associated with leaf N remobilization and the respective contributions of leaves and stems to N storage and remobilization during seed filling

Girondé, Alexandra; Etienne, Philippe; Trouverie, Jacques; Bouchereau, Alain; Le Cahérec, Françoise; Leport, Laurent; Orsel, Mathilde; Niogret, Marie-Françoise; Nesi, Nathalie; Carole, Deleu; Soulay, Fabienne; Masclaux-Daubresse, Céline; Avice, Jean-Christophe

doi:10.1186/s12870-015-0437-1

Table 2 NUE, NUtE, NUpE and NRE of Aviso and Oase under ample or low N supply

From: The contrasting N management of two oilseed rape genotypes reveals the mechanisms of proteolysis associated with leaf N remobilization and the respective contributions of leaves and stems to N storage and remobilization during seed filling

		Genotypes (G)				Source of variation
		Aviso		Oase		G	N		G x N
N treatment (N)		HN	LN	HN	LN	F _G	F _N	r	F _GxN
NUE^a		1.98 ± 0.06	3.00 ± 0.12^**	2.29 ± 0.11^#	2.86 ± 0.08^**	1.048	89.81^***	−0.90	7.13^*
NUtE^b		13.25 ± 0.66	20.27 ± 2.00^*	13.67 ± 0.50	23.45 ± 0.55^*	3.38	73.78^***	−0.90	1.10
NUpE^c (%; D70-D99)		45.86 ± 8.10	32.00 ± 5.30	81.57 ± 4.25^#	70.32 ± 7.88^#	8.72*	60.81^***	0.76	22.96^**
NRE^d (%)	Global
	0-99	59.12 ± 1.99	74.10 ± 3.22^**	52.83 ± 2.03	76.08 ± 4.10^**	0.70	55.28^***	−0.89	2.59
	Stepwise
	0-42	72.28 ± 3.48	86.62 ± 4.37^*	77.59 ± 4.52	83.26 ± 4.16	0.07	7.74^*	−0.60	1.45
	42-70	51.17 ± 1.46	77.80 ± 4.16^**	59.90 ± 2.92^#	75.65 ± 11.29	0.37	15.40^**	−0.73	1.01
	70-99	32.70 ± 2.75	39.96 ± 2.19	14.91 ± 1.13^#	27.70 ± 1.75^**#	71.99^***	31.99^***	−0.52	2.43

The plants were supplied with an ample (HN, 3.75 mM) or low (LN, 0.375 mM) nitrate concentration. D0 corresponds to the early bolting stage, D42 to pod formation, D70 to seed filling and D99 to mature seeds. The main source of variation is deduced from an ANOVA test where genotypes, N treatment, and genotype x N treatment interaction were tested. The resulting F values are presented below. The r values correspond to the correlation between N supply and NUE, NUtE, NUpE and NRE, respectively.
^a:NUE (Nitrogen Use Efficiency) corresponds to the mg of N in seeds per mg of the N amount in the whole plant relative to the seed DM (g) produced per g of total DM (including roots, pod walls and seeds).
^b:NUtE (Nitrogen Utilization Efficiency) corresponds to the g of seed DM produced per mg of N in shoots (including pod walls and seeds).
^c:NUpE (Nitrogen Uptake Efficiency) is estimated as the percentage of N derived from uptake in the total N (from remobilization and uptake) distributed to seeds between D70 and D99 (% of N distributed to seeds that derived from uptake).
^d:NRE (Nitrogen Remobilization Efficiency) is determined as the percentage of N stored in all source organs which is remobilized (the total remobilized N amount relative to the total N amount in all sources) between D0 (bolting stage) and final harvest (D99) or between D0 and D42, D42 and D70 and D70 and D99.
Asterisks represent significant differences between N treatments and hashes represent significant differences between the two genotypes (n = 4 plants; #,*= p < 0.05; **= p < 0.01; ***= p < 0.001).

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