Figure 4

Experimental validations of the newly-identified exons/ASVs. Shown in the figure are. RT-PCR products of the newly-identified isoforms that include the novel exons and the previously-annotated isoforms that exclude the novel exons in (A) O. sativa L. ssp. japonica cv. Nipponbare (designated as “Nip”) and (B) O. sativa L. ssp. indica cv. 93-11 (designated as “93-11”), O. rufipogon (designated as “Ruf”), O. nivara (designated as “Niv”), and O. glaberrima (designated as “Gla”). The black and gray arrows represent the newly-identified and previously-annotated isoforms, respectively.