Anchoring results for sequences with at least one positive pool. For both sequence datasets (GenomeZipper, Ae. tauschii), about half of sequences were anchored to BAC clones/physical map contigs. A particular BAC pool was considered positive if its reads covered at least 80% of particular sequence after read alignment to complete sequence set. A majority of the remaining sequences were not anchored either due to missing positive pool in one of the dimensions (no_positive_BAC) or no candidate clone overlap during the deconvolution (if more than one clone is positive; no_clone_ovlp).