Positive pool detection. Each individual pool was considered positive, if its reads covered at least 80% of particular sequence. a) Distribution of the number of sequences positive for given number of pools for GenomeZipper and Ae. tauschii sequence dataset. Only sequences with at least one positive pool were considered. b) Distribution of sequences with at least one positive pool among seven Ae. tauschii chromosomes. Note that a majority of sequences originate from chromosome 3D.