Hash size test for read alignment. Hash size was tested with respect to alignment sensitivity and time needed for analysis. a) Time needed to align 30x coverage of pool rP to 567 sequences in GenomeZipper dataset. b) The number of sequences positive for pool rP. Sequences covered with aligned reads at least from 80% of the length were considered as positive (i.e. represented in the pool). While hash size has no effect on alignment sensitivity, increasing hash size reduced time needed to align reads.