Coverage test for read alignment. Reads representing different sequencing depth of BAC pool rP from 1x to 50x were randomly selected from sequencing data and aligned to 567 sequences represented in GenomeZipper dataset. Sequences covered with aligned reads at least from 80% of the length were considered positive (i.e. represented in the pool). The curve reached plateau at about 30x coverage. The noise of the growth curve is due to random selection of reads for alignment.