Figure 5
Phenotypes of transgenic and wild-type Hongkong kumquats and cellular investigation. (A) Two-year-old flowering Hongkong kumquats (left, wild type; right, 35S:: CrtB, which represents transgenic Hongkong kumquat). (B) Phenotypes of flowers (left, wild type; right, 35S:: CrtB). (C) Phenotype of senescent leaf (top, wild type; lower, 35S:: CrtB). (D) Nucellar seedlings under light-grown for 60 d (left, wild type; right, 35S:: CrtB). (E, G, and I) Frozen sectioning investigation of the petal, leafstalk, and root of the wild-type Hongkong kumquat, respectively. (F, H, and J) Frozen sectioning investigation of the petal, leafstalk, and root of the transgenic Hongkong kumquat, respectively. (K, M, and O) Ultrastructural inspection of the petal, leafstalk, and root of the wild-type Hongkong kumquat, respectively. (L, N, and P) Ultrastructural inspection of petal, leafstalk, and root of the transgenic Hongkong kumquat, respectively. s, starch granules; p, plastoglobules; th, thylakoids; c, carotenoid crystal and characteristic internal membrane. The bars represent 10 μm in light microscopy and represent 1 μm in transmission electron microscopy.