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Table 3 RNA-Seq results from leaf sheaths confirmed by real-time PCR

From: Transcriptome analysis of nitrogen-starvation-responsive genes in rice

Locus ID

RNA-Seq log 2 (N-/N+)

RT-PCR log 2 (N-/N+)

Putative function

LOC_Os03g32230

4.35

2.25

Zinc finger protein

LOC_Os04g52090

2.32

1.56

Ethylene-responsive transcription factor 4

LOC_Os03g62200

2.26

2.66

Ammonium transporter protein

LOC_Os04g40410

2.25

4.89

High affinity nitrate transporter

LOC_Os02g08440

2.20

2.23

WRKY71

LOC_Os02g43790

1.89

1.27

Ethylene-responsive transcription factor 1A

LOC_Os03g55540

1.72

2.66

ZOS3-18 - C2H2 zinc finger protein

LOC_Os03g60560

1.67

1.08

Zinc finger protein ZAT12

LOC_Os03g60080

1.58

2.68

NAC domain-containing protein 67

LOC_Os01g50820

1.48

1.21

Transporter, major facilitator family

LOC_Os12g02440

1.47

1.89

WRKY transcription factor 46

LOC_Os12g07640

1.47

1.97

Myb-related protein

LOC_Os11g03540

1.46

1.63

Ethylene-responsive transcription factor

LOC_Os05g03900

1.43

1.85

WRKY109

LOC_Os06g41100

1.32

2.32

bZip, transcription factor

LOC_Os07g12340

1.32

1.38

NAC domain-containing protein 67

LOC_Os02g43330

1.30

1.43

Homeobox-associated leucine zipper

LOC_Os02g53130

1.26

2.12

Nitrate reductase

LOC_Os01g53220

1.26

−1.40

Heat stress transcription

LOC_Os02g43170

1.20

3.19

B-box zinc finger family protein

LOC_Os04g50770

1.20

2.21

MYB-related protein Zm1

LOC_Os04g45810

1.13

2.45

Homeobox-leucine zipper protein HOX22

LOC_Os02g49840

1.08

3.32

MADS-box transcription factor 57

LOC_Os01g54600

1.07

2.87

WRKY13

LOC_Os09g28354

1.07

1.07

Heat stress transcription factor B-1

LOC_Os04g43070

1.02

1.72

Ammonium transporter protein

LOC_Os03g42200

−2.00

−1.02

Dof zinc finger domain containing protein

LOC_Os03g08620

−1.60

−2.38

B3 DNA binding domain-containing protein

LOC_Os04g44440

−1.59

0.88

TCP family transcription factor

LOC_Os01g74540

−1.56

1.00

GATA zinc finger domain-containing protein

LOC_Os05g34110

−1.42

0.97

Homeodomain-related

LOC_Os10g42490

−1.26

−2.03

Homeobox and START domain-containing proteins

LOC_Os02g39140

−1.06

1.01

Helix-loop-helix DNA-binding domain containing protein

LOC_Os06g43220

−1.04

−2.12

AP2 domain-containing protein

  1. Expression ratios for RNA-Seq data were calculated with the DESeq program. All ratios are presented as Log2N-deficient/N-sufficient. Negative values indicate that expression was reduced under N-starvation.