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Table 1 Identification of differentially expressed proteins in the roots of Rangpur lime and ‘Sunki Maravilha’ mandarin subjected to water deficit

From: Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit

ID spot a Identified protein reference organism b Accession number c Mascot score/ P -value d Mr Theor/Exp e pI Theor/Exp f Expression level g A B C D Fold change (P < 0.05) h RL Sk
8 Epidermis-specific secreted glycoprotein EP1-like Citrus sinensis XP_006477736 155/1e-08 48.8/18 6.26/6.92 −3.81 np
10 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial-like Citrus sinensis XP_006475040 67/4e-15 51.1/42 9.13/6.55 −2.11 2.17
13 epidermis-specific secreted glycoprotein EP1-like Citrus sinensis XP_006477736 130/0.0 48.8/59 6.27/6.61 −1.82 1.50
15 miraculin-like protein 1 Citrus maxima AEK31192 176/1e-20 18.9/15 8.18/5.80 2.32 1
16 Germin-like protein subfamily T member 2-like Citrus sinensis XP_006477534 235/2e-169 25.9/24 5.74/5.35 −1.65 1.2
18 Germin-like protein subfamily T member 2-like Citrus sinensis XP_006477534 235/2e-169 25.9/24 5.74/5.15 −1.57 -1.38
19 Germin-like protein subfamily T member 2-like Citrus sinensis XP_006477534 235/2e-169 25.9/27 5.74/5.96 −1.39 2.27
20 Nucleoside diphosphate kinase Citrus sinensis XP_006464834 60/2e-09 16.3/13 5.91/6.21 Np 1
21 2-phospho-D-glycerate hydrolase Citrus trifoliata ADD12953 911/2e-18 47.77/39 5.42/5.57 −1.86 1.56
25 Annexin D2 Arabidopsis thaliana NP_174810 160/6e-173 36.20/38 5.21/5.83 1.60 2.47
26 Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana NP_175610 189/4e-14 48.20/50 6.08/5.79 −1.47 1
27 Lipase class 3 family protein Arabidopsis thaliana NP_567515 150/0.0 59.06/59 9.33/5.86 −1.33 ∞
29 TIR-NBS-LRR type disease resistance protein Citrus trifoliata AAN62351 93/2e-37 41.4/30 7.10/4.91 3.17 -1.62
34 Germin-like protein 3–3 like Citrus sinensis XP_006477531 222/3e-31 43.3/24 5.73/6.07 1 -2.79
38 Germin-like protein 3–3 like Citrus sinensis XP_006477531 222/3e-31 43.3/27 5.73/6.45 1 -3.45
40 Glyoxalase Theobroma cacao XP_007026102 413/4e-144 27.06/33 6.52/5.64 1.73 -3.26
41 Mitochondrial malate dehydrogenase Citrus sinensis AET22414 285/4e-98 30.89/34 5.2/6.10 −1.71 ∞
42 Chitinase Citrus sinensis CAA93847 58/0.0045 32.45/35 5.06/4.80 3.44 np
46 Heat shock protein 70 Arabidopsis thaliana CAA05547 77/0.0 71.4/46 5.14/6.32 1.64 1.76
48 2-phospho-D-glycerate hydrolase Citrus trifoliata ADD12953 911/0.0 47.77/46 5.42/5.45 2.63 1
49 Methyl-CPG-binding domain 6 protein Arabidopsis thaliana NP_200746 150/2e-43 24.44/49 9.03/6.0 1.35 2.07
51 Annexin 1 Theobroma cacao NP_174810 109/0.0 35.8/48 6.34/5.72 2.16 np
54 2-phospho-D-glycerate hydrolase Citrus trifoliata ADD12953 911/0.0 47.77/51 5.42/5.7 −1.94 -2.53
55 Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana NP_175610 545/0.0 54.4/56 5.94/5.9 1.42 ∞
57 Mitochondrial processing peptidase subunit alpha-1 Arabidopsis thaliana NP_175610 545/0.0 60.79/57 7.06/6.26 1.83 1.82
59 Annexin 1 Theobroma cacao EOY16019 566/0.0 35.8/36.1 6.34/5.42 np 2.09
60 Annexin 1 Theobroma cacao EOY16019 566/0.0 35.8/36.1 6.34/5.42 np 2.38
61 Lactoylglutathione lyase Citrus X paradisi CAB09799 598/0.0 32.63/66 5.28/6.05 −1.81 1.66
63 Lactoylglutathione lyase S-transferase Ricinus communis XP_002518470 52/8e-146 31.5/66 7.63/5.99 −1.90 1.65
70 Peroxidase Citrus maxima ABG49115 517/0.0 37.88/44 4.52/6.10 −3.21 1
71 Histone ubiquitination proteins group Populus trichocarpa XP_002302510 188/0.0 48.1/67 5.56/5.71 np −3.61
72 Acyl-CoA -N-acetyltransferase Arabidopsis thaliana NP_196882 46.4/7e-29 20.39/22 7.8/5.48 np 1
75 5-formyltetrahydrofolate cyclo-ligase Arabidopsis thaliana NP_565139 355/1e-119 39.55/39 9.41/6.63 np −2.20
79 2-phospho-D-glycerate hydrolase Citrus trifoliata ADD12953 62.6/0.0 47.78/34 5.54/6.39 −2.68 np
93 mRNA-capping enzyme Arabidopsis thaliana NP_974263 74.4/0.0 78.7/75.57 6.74/5.52 Np 1
94 Annexin D2 Citrus sinensis CAB09799 116/4e-122 19.8/36 5.30/5.16 −1.79 np
100 ATP synthase beta subunit Citrus macroptera ABM74441 69.4/3e-152 37.07/58 5.01/5.74 −2.68 -4.18
113 2-dehydro-3-deoxyphosphooctonate aldolase Medicago truncatula ABN05924 427/2e-13 31.9/28 6.61/4.91 np ∞
116 Fructokinase Oryza sativa A2WXV8 70/1e-34 30.3/87 5.50/6.19 np ∞
154 Heat shock protein-70 cognate protein Arabidopsis thaliana NP_176036 73/0.0 71.4/65 5.10/5.27 ∞ np
194 F-box family protein Vitis vinifera XP_002279122 414/4e-139 47.2/55 9.4/6.39 −1.57 np
196 Fructokinase Citrus unshiu AAS67872 219/2e-71 37.5/36 5.11/4.97 −2.54 1.64
202 Putative mitochondrial processing peptidase Arabidopsis thaliana BAE98412 202/0.0 51.53/53 5.71/6.49 2.88 -1.95
205 Putative L-galactose dehydrogenase Citrus unshiu ADV59927 294/1e-18 37.62/25 6.03/6.23 1.75 ∞
207 Putative mitochondrial processing peptidase Arabidopsis thaliana BAE98412 480/0.0 51.53/55 5.71/6.33 1.56 1.47
  1. aSpot ID corresponding to the position in the 2D gel illustrated in Figure 1. bProtein accession number according to the NCBI database (http://www.ncbi.org). cBest matching protein identified by pBLAST analysis of the non-redundant (NCBInr) database. dMascot score P value of the homology between citrus proteins and orthologous, homologous, or paralogous proteins, as annotated in NCBInr. eTheoretical and experimental masses (KDa) of identified proteins. fTheoretical and experimental pIs of identified proteins. gExpression levels, presented as the % normalised volume, in the control and water deficit-stressed roots. Vertical bars indicate the mean ± SE. Rangpur lime: (A) control; and (B) water deficit. ‘Sunki Maravilha’: (C) control; and (D) water deficit. hFold change (water deficit-treated normalised volume/control normalised volume): bold = increased protein abundance; underlined = decreased protein abundance; italics = no significant difference; np = protein not found in gel; ∞ = present in one treatment in the genotype.
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