Figure 11
qRT-PCR confirms the differential expression of eight rice histone chaperone genes under various abiotic stress conditions. Abiotic stress-responsiveness of the genes which were found to show altered expression in one or more abiotic stresses via microarray-based expression analysis was further studied by qRT-PCR in both stress-sensitive and salinity-tolerant genotypes. Bar graphs depict mean fold change (log2 scale) in expression of abiotic stress-regulated histone chaperones in contrasting genotypes – moderately stress sensitive IR64 (A), and salt stress tolerant Pokkali (B) under various abiotic stress conditions – ABA, drought, heat, oxidative, and salinity, as obtained via expression analysis using qRT-PCR. Error bars show ± standard deviation, n = 3. Names of the genes have been mentioned at the top of each of the bar graphs. Each of the eight genes were found to be differentially regulated under at least one of the stress conditions in both the genotypes, with most of them exhibiting multiple stress-responsive nature.