Abscisic acid and salicylic acid level in different barley genotypes. Seven-day-old primary barley leaves were harvested from cultivar Ingrid, Himalaya, Steptoe, the backcross line BCIngridmlo5, and the mutant Az34, respectively. Samples consisting of five leaves were analysed by HPLC-MS-MS for ABA (A) or SA (B) content. Means and standard deviations for three samples harvested in a single experiment are shown. The experiment was repeated twice for ABA and once for SA determination with similar results.