Figure 1From: Abscisic acid negatively interferes with basal defence of barley against Magnaporthe oryzae Microscopic evaluation of the infection of M. oryzae on barley. Primary leaves of barley cultivar Ingrid were inoculated with a spore solution of M. oryzae isolate TH6772 (200,000 conidia mL−1) seven days after sowing. Leaves were harvested at 72 h p.i. and placed in 25% acetic acid in ethanol (v/v) until bleached. Thereafter, leaves were analyzed in water by bright field (A, B, D and E) or epi-fluorescence (C, F, G, H and I) microscopy. Category designations and labels correspond to the quantitative evaluation in Figures 2, 3 and 5C. app: appressorium; sechy: secondary hyphae; con: conidium; pap: papilla; gt: germ tube; epiHR: epidermal hypersensitive response; rmes: round-shaped mesophyll cells; cmes: collapsed mesophyll cells.Back to article page