Microscopic evaluation of the infection of
on barley. Primary leaves of barley cultivar Ingrid were inoculated with a spore solution of M. oryzae isolate TH6772 (200,000 conidia mL−1) seven days after sowing. Leaves were harvested at 72 h p.i. and placed in 25% acetic acid in ethanol (v/v) until bleached. Thereafter, leaves were analyzed in water by bright field (A, B, D and E) or epi-fluorescence (C, F, G, H and I) microscopy. Category designations and labels correspond to the quantitative evaluation in Figures 2, 3 and 5C. app: appressorium; sechy: secondary hyphae; con: conidium; pap: papilla; gt: germ tube; epiHR: epidermal hypersensitive response; rmes: round-shaped mesophyll cells; cmes: collapsed mesophyll cells.