Figure 3
S. littoralis oral secretion (OS) induces JA accumulation independent of the FAC content of the OS. A: The five most abundant FAC compounds were measured by HPLC-MS for all HAEs and control used in this study. Water; oral secretion from S. littoralis (OS Sl ); OS Sl with fatty acid amino acid conjugates (FAC) removed (FAC-free OS Sl ) using an ion exchange column; C18:3-Glu and oral secretion from M. sexta (OS Ms ). Each shading represents a specific FAC compound. Y-axis shows the peak area of the target molecular ion of each compound. For each HAE, six replicates were used. Error bar indicates the standard deviation. “nd” refers to samples in which an FAC was not detected. (B) The induced JA accumulation in N. attenuata induced by wounding + oral secretion of S. littoralis. Y-axis shows the JA concentration at 30 minutes after induction and axis refers to different HAEs that were added to leaf punctures. Letters indicates the significance of differences (p < 0.05, post hoc Tukeyhonest significant test after ANOVA).