Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea

Table 2 Assembly statistics of high quality reads

Parameters	K-mer
Parameters	21	27	33	39	45	51	57	63
Number of contigs	204991	248954	*262233*	220102	170941	134378	99899	68700
Assembly length (million bp)	69.8	96.1	111.1	*111.6*	104.4	91.9	72.4	47.0
Minimum transcript length (bp)	100	100	100	100	100	100	100	100
Maximum transcript length (bp)	10071	*12248*	11901	11782	11856	9105	8870	7678
Average transcript length (bp)	340	385	423	506	610	683	*724*	684
N50 (bp)	665	832	989	1144	1265	*1301*	1241	1057

Pooled high quality reads were assembled at various K-mers using SOAPdenovo. For each of the K-mer various assembly parameters (such as number of contigs, assembly length, minimum, maximum and average transcript length and N50) were evaluated. The maximum value for each of the parameter in their respective k-mers has been italicized.

ISSN: 1471-2229