Table 1 Filtering of raw reads obtained through high throughput sequencing of RNA-Seq libraries
Category | BC | BHS | BDS |
|---|---|---|---|
Number of reads | Number of reads | Number of reads | |
(Percentage) | (Percentage) | (Percentage) | |
Raw reads | 77926818 (100%) | 65644688 (100%) | 40181314 (100%) |
Adapter contaminated | 155835 (0.2%) | 4872907 (7.4%) | 889239 (2.2%) |
Low quality | 11662189 (15.0%) | 9706889 (14.8%) | 3747523 (9.3%) |
High quality paired reads | 58438630 (75.0%) | 41320578 (62.9%) | 32342960 (80.5%) |
High quality unpaired reads | 7670164 (9.8%) | 9744314 (14.8%) | 3201592 (8.0%) |
Total high quality reads | 66108794 (84.8%) | 51064892 (77.8%) | 35544552 (88.5%) |