Figure 6From: Global insights into high temperature and drought stress regulated genes by RNA-Seq in economically important oilseed crop Brassica juncea Relative abundance of selected transcripts as determined by qPCR. Expression profiling of a few differentially regulated transcripts was performed using quantitative real time PCR. The relative abundance (Y-axis) was calculated using ΔΔCt method. B. juncea seedlings were subjected for varied durations to either high temperature stress (BHS) at 42°C for 30 min, 2 h and 4 h or drought stress (BDS) by using 300 mM mannitol for 1 h, 3 h and 6 h. The mean of three independent biological replicates is presented.Back to article page