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Figure 5 | BMC Plant Biology

Figure 5

From: Label-free nanoUPLC-MSE based quantification of antimicrobial peptides from the leaf apoplast of Nicotiana attenuata

Figure 5

Linear dynamic range of nanoUPLC − MS E measurements of AMPs. To determine the linear dynamic range of quantification, the calculated peptide amounts [fmol/column] from 3–5 technical replicates were plotted against the corresponding amount of BSA in the sample (50–500 fmol); BSA was linear in the full range tested. (A) Linear regression (R2) shown for the high abundant AMPs (LEA, ICE and DEF2). (B) Linear regression (R2) shown for the low abundant AMPs (VRD, FAB and DEF1).

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BMC Plant Biology

ISSN: 1471-2229

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