Figure 4
Comparison of peptide abundance calculated from LC-MS E data of different transgenic N. attenuata lines. Intercellular fluid (ICF) was extracted with MES buffer (pH 5.5) and desalted using reversed phase cartridges. The samples were analyzed by nanoUPLC-MSE and the peptide abundance calculated based on the relation between the averages of the intensity of the three most intense peptides of the internal standard (BSA) to the peptides of interest [38]. Peptide abundances are shown as pmol per g fresh mass (FM) ± SEM from 3 biological replicates per genotype (6 biological replicates for DEF2, ICE and LEA lines); n.d. = not detected.