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Figure 3 | BMC Plant Biology

Figure 3

From: Label-free nanoUPLC-MSE based quantification of antimicrobial peptides from the leaf apoplast of Nicotiana attenuata

Figure 3

Comparison of the MALDI-TOF mass spectra acquired from the intercellular fluid of WT and transgenic ICE and LEA lines. ICF was extracted with citrate buffer (pH 3.0), desalted by ultrafiltration (VWR 3K columns) and analyzed in linear ion mode in the mass range 1–10 kDa. Peaks within the mass ranges of the expressed peptides are highlighted. The inset shows the MALDI-TOF MS analysis of the supernatant from WT and LEA lines (35 mL concentrated by Amicon 3K columns).

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