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Figure 2 | BMC Plant Biology

Figure 2

From: Label-free nanoUPLC-MSE based quantification of antimicrobial peptides from the leaf apoplast of Nicotiana attenuata

Figure 2

Schematic representation of the workflow used for sample preparation of antimicrobial peptides (AMPs). Intercellular fluid (ICF) was extracted by vacuum infiltration and desalted using reversed phase solid phase extraction cartridges (SPE). The samples were spiked with bovine serum albumin (BSA) which served as internal standard, tryptically digested and analyzed by nanoUPLC-MSE. Final peptide quantity was calculated and expressed as pmol per g fresh mass (FM).

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