Skip to main content

Table 2 Specific flour protein types that increased or decreased in relative amounts when non-transgenic and transgenic plants were grown with post-anthesis fertilizer

From: Silencing of omega-5 gliadins in transgenic wheat eliminates a major source of environmental variability and improves dough mixing properties of flour

 

%Change with Fertilizer 1

Protein Type 2

Control

35b

45a

HMW-GS By9

32.0

35.2

25.8

LMW-GS Bu-11 (m-type)

−13.4

−17.9

−21.2

LMW-GS Bu-18 (m-type)

−5.3

−9.6

−21.4

LMW-GS [GenBank: AAB48469] (i-type)

13.5

24.7

17.7

Omega-1,2 chain-terminating gliadin3

89.2

93.3

26.3

Alpha gliadin Bu-5

49.8

41.7

30.5

Alpha gliadin Bu-11

32.6

32.0

23.6

Alpha gliadin Bu-12

38.1

38.5

27.1

Alpha gliadin Bu-14

32.1

32.6

28.3

Alpha gliadin Bu-27

24.0

−18.8

8.6

Alpha gliadin Bu-BQ807130

29.5

11.6

17.9

Omega-5 gliadin

70.7

3.3

−3.9

Omega-1,2 gliadin D34

31.6

26.9

4.3

Omega-1,2 gliadin Bu-D54

79.5

66.0

19.1

Secalin-like omega gliadin

31.5

24.5

14.1

Triticin

15.4

15.5

21.3

Farinin Bu-1

−29.9

−39.7

−43.1

Farinin Bu-2

−30.8

−31.0

−28.1

Purinin Bu-1

−11.9

−25.4

−26.9

Purinin Bu-2

−12.8

−22.4

−32.1

Purinin Bu-3

−7.3

−11.0

−24.4

Serpin Bu-1

49.6

55.9

31.6

Serpin Bu-2

103.7

118.2

69.3

Serpin Bu-3

54.3

63.8

60.3

Serpin Bu-4

56.3

60.2

53.2

Serpin Bu-5

50.7

51.1

47.2

Serpin Bu-7

26.3

11.9

16.2

WMAI Bu-1

−24.8

−26.0

−31.1

WDAI Bu-1

−17.9

−25.2

−25.8

WDAI Bu-4

−27.4

−41.6

−38.8

WTAI CM1

−17.9

−26.1

−31.1

WTAI CM2

−26.0

−34.8

−30.9

WTAI CM3

−26.0

−34.0

−38.9

WTAI CM16

−27.7

−35.7

−37.0

WTAI CM17

−30.6

−39.7

−39.8

WCI

−30.0

−38.1

−39.6

CMX1/CMX3

−23.0

−28.7

−31.0

WASI

−29.1

−25.8

−28.0

  1. 1Protein types that either increased or decreased at least 20% in response to post-anthesis fertilizer are indicated in bold. Changes greater than 20% that were not deemed to be significant by ANOVA are shown in bold italics.
  2. 2Only protein types that changed in response to fertilizer in at least one line are shown.
  3. 3Protein has an amino acid sequence similar to an omega-1,2 gliadin but contains one cysteine that enables it to be linked into the glutenin polymer.
  4. 4Two distinct protein types were distinguished for omega-1,2 gliadins. Percent changes of total omega-1,2 gliadins with fertilizer were 47.7, 39.3 and 7.6 for the non-transgenic, 35b and 45a, respectively.