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Figure 4 | BMC Plant Biology

Figure 4

From: AtROP1 negatively regulates potato resistance to Phytophthora infestans via NADPH oxidase-mediated accumulation of H2O2

Figure 4

The identification of positive potato transgenic lines. A. Southern blot analysis of DN-AtRop1 transgenic lines with GFP probe. About 15 μg of the genomic DNA was digested with EcoR I and Sac I, electrophoresed on a 1.0% agarose gel, and blotted on to a positively charged nylon membrane. C: untransformed control plant, E: potato transformed with the empty vector p1300-221, 1–4: DN-AtRop1 potato transgenic lines (6, 7, 8, 11). B. Western blots analysis of the protein expression level of DN-AtROP1 in potato transgenic lines using an anti-GFP monoclonal antibody. A: positive control with only GFP protein, C: untransformed control plant. 1–4: DN-AtRop1 potato transgenic lines (6, 7, 8, 11).

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