Figure 5

Protein and mRNA expression levels of differentially expressed proteins spots from wild-type (WT) and PGMS mutant (MT) cotton ( Gossypium hirsutum L.). (A) Magnified view of six protein spots from the total proteome. (B) qRT-PCR analysis of the transcripts of the corresponding genes. The mRNA expression value of each gene was normalized to that of 18S rRNA, the reference gene, followed by normalization against the tetrad stage of the WT. Data represent the mean and standard deviation from three replications. *P < 0.05; **P < 0.01 according to Student’s t-test of the value at the same stage. The exact values were shown in Additional file 6. CHS (spot 137), chalcone synthase; EACPR (spot 145), enoyl-[acyl-carrier-protein] reductase; PME1 (spot 173), pectinesterase PPME1; APX1 (spot 126), l-ascorbate peroxidase 1; RPT3 (spot 63), 26S protease regulatory subunit 6B and PAB2 (spot168), proteasome subunit beta type-1.