Figure 1

3D structures of CenH3 proteins from a single species in each genus. a. Brassica napus (AC8). b. Oryza australiensis isolate (EE). c. Gossypium raimondii (D5). The secondary protein structures are depicted below each 3D model containing nonsynonymous SNPs within each genus mapped to the secondary structure of the CenH3 protein. The different shades of blue signify the borders of each protein domain; α signifies an alpha helix and L signifies a loop. Black rectangles signify nonsynonymous SNPs, while yellow rectangles signify nonsynonymous SNPs under positive selection (Figure 3). The N terminus was the primary site of length variation among the three genera and the long black rectangle in the N terminus of the Brassica CenH3 signifies gaps in the alignment among Brassica species.