Marker trait association analysis scans using Fisher’s exact test Vertical axis represents -log10 (P) values of the P-value of the marker trait association. The peaks above minimum threshold of 2 (P-value = 0.03) can be considered as significantly associated. The colours blue and red were used to differentiate between chromosomes (1H-7H). (A) Chromosome-wise plot and (B) genome-wide manhattan plot of chromosome 5HL derived from marker-trait association (MTA) analysis using Fisher’s exact test on 2 X 2 count table for seedling resistance to Puccinia hordei pathotype 253P- (binary scoring data) in the CI9214/Stirling doubled haploid population using 4, 500 DArT-seq markers. The –log10 of P-values were plotted against the positions on the physical Bowman genome assembly . The peaks above minimum threshold of 2 (P-value = 0.03) can be considered as significantly associated.