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Figure 6 | BMC Plant Biology

Figure 6

From: Multiscale investigation of mealiness in apple: an atypical role for a pectin methylesterase during fruit maturation

Figure 6

MdPME2 cloning and validation by semi-quantitative RT-PCR. A Analysis of MDP0000222620 sequence annotated on Malus x domestica genome [22]. At1g53830 is the Arabidopsis thaliana most similar sequence. MDP0000245813 is the complete sequence identified from the 3’ RACE analysis. All 3 protein sequences are characterized by the presence of a PRO domain region (PF04043) followed by a putative processing motif [33],[69]. The PME domain (PF01095) of MDP0000222620 is incomplete. B Confirmation of the cloned gene expression profiles with 2 PME specific primer sets by semi-quantitative RT-PCR for 30 cycles. M20 genomic DNA (DNAg) was used as positive control. Genes expression were studied for pair 2 (M20/M74) during complete fruit kinetic. MDP0000245813 could be amplified with clear difference between mealy (M) and non-mealy (N) genotypes, while there was no amplification with MDP0000222620, and GADPH expression remained at a relatively constant level.

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