Figure 7

MAPK activation profile in the T-DNA insertion pbl1 , bik1 or pbl1bik1 double mutants. 14-day-old liquid-grown seedlings were equilibrated in 1.5 ml of fresh MS medium for ~24 h, and elicited by adding 0.5 ml of media containing a 4-fold concentrated stock of the indicated MAMP/DAMPs. Samples were collected at the indicated time points (min) after treatment and proteins were extracted for immunoblotting to detect phosphorylated (i.e. activated) forms of the MAPKs. Amido black staining of the nitrocellulose membranes was used to estimate equal loading.